Coding

Part:BBa_K112807:Design

Designed by: Jin Huh   Group: iGEM08_UC_Berkeley   (2008-10-31)


[T4 antiholin]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 220


Design Notes

Higher constitutive expression of antiholin compared to the expression of holin is required for the stable lysis device.


Source

Enterobacteria phage T4 http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=29366675&from=59202&to=59495&view=gbwithparts

References